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Anti-HER2 VHH Targeted Fluorescent Liposome as Bimodal Nanoparticle for Drug Delivery and Optical Imaging

[ Vol. 16 , Issue. 4 ]

Author(s):

Sepideh Khaleghi, Fatemeh Rahbarizadeh* and Shahryar K. Nikkhoi   Pages 552 - 562 ( 11 )

Abstract:


Objectives: The aim of this study was to formulate fluorescent-labeled targeted immunoliposome to visualize the delivery and distribution of drugs in real-time.

Methods: In this study, fluorescent-labeled liposomes were decorated with anti-HER2 VHH or Herceptin to improve the monitoring of intracellular drug delivery and tumor cell tracking with minimal side effects. The conjugation efficiency of antibodies was analyzed by SDS-PAGE silver staining. In addition, the physicochemical characterization of liposomes was performed using DLS and TEM. Finally, confocal microscopy visualized nanoparticles in the target cells.

Results: Quantitative and qualitative methods characterized the intracellular uptake of 110±10 nm particles with near 70% conjugation efficiency. In addition, live-cell trafficking during hours of incubation was monitored by wide-field microscopy imaging. The results show that the fluorescent- labeled nanoparticles can specifically bind to HER2-positive breast cancer with minimal off-target delivery.

Conclusion: These nanoparticles can have several applications in personalized medicine, especially drug delivery and real-time visualization of cancer therapy. Moreover, this method also can be applied in the targeted delivery of contrast agents in imaging and thermotherapy.

Keywords:

Fluorescent liposome, HER2, live-cell imaging, targeted delivery, VHH, wide-field microscopy.

Affiliation:

Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran



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